Colette A. Sacksteder, Ph.D.
Expertise
Colette A. Sacksteder
Dr. Sacksteder's research interests focus on the use of a systems biology approach to identify critical mechanisms that facilitate adaptive stress responses in cells. To accomplish this, global proteomic methods are being used to identify protein-protein interaction networks that respond to cellular stress, with the goal of generating predictive cellular models. Important to this approach, is the identification of underlying switching mechanisms that modulate changes in cellular signaling and metabolism. This is accomplished by measuring protein conformational changes, primarily through the use of FT-IR spectroscopy whose sensitivity permits high-throughput measurements. Combined with isotopic labeling techniques FT-IR can be used to explore protein-protein interactions and structural effects of protein modification. Using these methods the modulation of protein interactions by oxidative stress is being assessed within a mouse myoblast model system.
Previous research experience in photosynthesis ranges from the study of the protein environment on electron transfer in cyanobacterial Photosystem I to the investigation of stress on the electron transfer process in intact plants.
Education
- Ph.D., Biochemistry, Washington State University, 2000
- B.S., Physics, Washington State University, 1995
Honors and Awards
- Paul D. Boyer & James B. Peter Postdoctoral Research Award , University of Minnesota, 2003
- Loyal Davis Fellowship, Institute of Biological Chemistry, Washington State University, 1998
- National Institutes of Health Biotechnology Training Grant Fellowship, 1995-2000
- Distinguished Student Award, College of Science, Washington State University, 1995
- Phi Beta Kappa Scholarship, Washington State University, 1994
Selected Publications
Sacksteder CA, SL Bender, and BA Barry. 2005. "Role for Bound Water and CH-π Aromatic Interactions in Photosynthetic Electron Transfer." Journal of the American Chemical Society.
Sacksteder CA, M Jacoby, and DM Kramer. 2002. "A portable, non-focusing optics spectrometer (NoFOSpec) for measurements of steady-state absorbance changes in intact plants." Photosynthesis Research 70:231-240.
Kim S, CA Sacksteder, KA Bixby, and BA Barry. 2001. "A reaction-induced FT-IR study of cyanobacterial Photosystem I." Biochemistry 40:1226-1237.
Sacksteder CA and BA Barry. 2001. "Fourier transform infrared spectroscopy: A molecular approach to an organismal question." Journal of Psychology 37:197-199.
Sacksteder CA and DM Kramer. 2000 "Dark interval relaxation kinetics of absorbance changes as a quantitative probe of steady-state electron transfer." Photosynthesis Research 66:145-158.