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Margaret R. Romine, Ph.D.


Margaret Romine
Margaret Romine

Genetic characterization of microbial respiratory pathways, signal transduction pathways, aromatic catabolic metabolism, and siderophore biosynthesis; intermediate scale DNA sequence assembly; microbial genome annotation; utilization of genome sequence data to develop research hypotheses, design experiments to test these hypotheses, and to interpret data from microarray and high throughput proteome analyses; development of genetic tools for fluorescent labeling of microbial cells as well as monitoring promoter activity and protein localization.

Research Focus

Dr. Romine's current research is primarily focused on studying the respiratory metabolism of Shewanella oneidensis MR-1. As a participant in a multi-institution project funded by DOE's Genomics: Genomes to Life (GTL) Program, she is charged with coordinating and conducting research on both updating the genome annotation and generating targeted genes deletions for 40 predicted c-type cytochromes encoded by MR-1. In order to facilitate the development of new tools for image analysis of Shewanella behavior, she participates in joint projects with Dr. Sunney Xie (Harvard) who is developing a genome wide transcriptional reporter library in MR-1, Dr. Shimon Weiss (UCLA) who is developing a novel method to map regulator binding to intact genomic DNA, and Dr. Gayla Orr (PNNL) who is evaluating a Fret-based method for analysis of protein-protein interactions in MR-1. She is also interested in the behavior of bacteria in mulitcellular communities or biofilms. In order to branch into this area, she is working with a group of scientists at PNNL to develop novel approaches for studying metabolite production, surface corrosion, and biofilm structural properties using acoustic, NMR, two photon and confocal instrumentation coupled to various fluorescent reporters.


  • Ph.D., Microbiology, University of Texas, 1988
  • B.S., Microbiology, University of Illinois, 1982

Honors and Awards

  • R&D100 Award 1997 for RubberCycle technology
  • Discover Magazine Environmental Technology Finalist 1996 for RubberCycle technology

Professional Affiliations

  • American Society of Microbiology

Selected Publications

Pinchuk GE, DA Rodionov, C Yang, X Li, AL Osterman, E Dervyn, OV Geydebrekht, SB Reed, MF Romine, FR Collart, JH Scott, JK Fredrickson, and AS Beliaev. 2009. "Genomic reconstruction of Shewanella oneidensis MR-1 metabolism reveals a previously uncharacterized machinery for lactate utilization." Proc. Natl. Acad. Sci. U. S. A. 106(8):2874-2879.

Fredrickson JK, MF Romine, AS Beliaev, JM Auchtung, ME Driscoll, TS Gardner, KH Nealson, AL Osterman , G Pinchuk, JL Reed, DA Rodionov, JL Rodrigues, DA Saffarini, MH Serres, AM Spormann, IB Zhulin, and JM Tiedje. 2008. "Towards environmental systems biology of Shewanella." Nat. Rev. Microbiol. 6(8):592-603.

Gupta NJ, J Benhamida, V Bhargava, D Goodman, E Kain, I Kerman, N Nguyen, N Ollikainen, J Rodriguez, J Wang, MS Lipton, M Romine, V Bafna, RD Smith, PA Pevzner. 2008. "Comparative proteogenomics: combining mass spectrometry and comparative genomics to analyze multiple genomes." Genome Res. 18(7):1133-1142.

Romine MF, TS Carlson, AD Norbeck, LA McCue, and MS Lipton. 2008. "Identification of mobile elements and pseudogenes in the Shewanella oneidensis MR-1 genome." Appl. Environ. Microbiol. 74(10):3257-3265.

Pinchuk GE, C Ammons, DE Culley, SM Li, JS McLean, MF Romine, KH Nealson, JK Fredrickson, and AS Beliaev. 2008. "Utilization of DNA as a sole source of phosphorus, carbon, and energy by Shewanella spp.: ecological and physiological implications for dissimilatory metal reduction." Appl. Environ. Microbiol. 74(4):1198-1208

Contact Information

Systems Biology at PNNL

Research & Capabilities