Brian S. Hooker, Ph.D.
Experience
Brian S. Hooker
Dr. Hooker is currently the team leader for the High Throughput Biology Team and Operations Manager of the DOE Genomics: Genomes to Life (GTL) Center for Molecular and Cellular Systems. Dr. Hooker also manages applied plant and fungal molecular biology projects including development of plant-based biosensors and transgenic production systems for human pharmaceutical proteins and industrial enzymes. Dr. Hooker's previous pursuits involve microbial kinetics and transport mathematical modeling, design, development, and support for the demonstration of in situ/ex situ biological destruction of chlorinated organic hydrocarbons. Specific accomplishments include:
- Development of the tP4 (transgenic Plant Protein Production Platform) technology
- Development of the RT3D bioremediation/natural attenuation software package.
Education
- Ph.D., Biochemical Engineering, Washington Sate University, Pullman, WA, 1990
- M.S., Biochemical Engineering, Washington State University, Pullman, WA, 1988
- B.S., Chemical Engineering, California State Polytechnic University, Pomona, CA, 1985
Honors and Awards
- Entrepreneurial Award, Battelle, 2001
- Federal Laboratory Consortium Recognition Award, "Reactive Transport in 3-Dimensions," 1999
- Outstanding Performance Award, Pacific Northwest National Laboratory, "Reactive Transport in 3-Dimensions," 1999
- Outstanding Performance Award, Pacific Northwest National Laboratory, "Demonstration of In Situ Bioremediation of Carbon Tetrachloride," 1997
- Licensed Professional Engineer (State of Washington), 1995-2005
- Coinventor on 4 issued patents
Selected Publications
Lin CT, LM Markillie, TC Squier, BS Hooker, and L Shi. 2005. "Expression of recombinant decaheme cytochrome MtrA in Shewanella oneidensis MR-1 by a directional TOPO® cloning-based system." BioTechniques 38(2):297-9.
Markillie LM, CT Lin, JN Adkins, DL Auberry, EA Hill, BS Hooker, PA Moore, RJ Moore, L Shi, HS Wiley, and V Kery. 2005. "A simple protein complex purification and identification method for high throughput mapping of protein interaction networks." Journal of Proteome Research
Gao J, BS Hooker, and DB Anderson. 2004. "Expression of Functional Human Coagulation Factor XIII A-domain in Plant Cell Suspensions and Whole Plants." Protein expression and purification 37(1):89-96.
Dai Z, BS Hooker, DB Anderson, and SR Thomas. 2000. "Improved plant-based production of E1 endoglucanase using potato:expression optimization and tissue targeting." Molecular Breeding 6(3):277-285.
Dai Z, BS Hooker, RD Quesenberry, and J Gao. 1999. "Expression of Trichoderma reesei exo-cellobiohydrolase I (CBH I) in transgenic tobacco leaves and calli." Applied Biochemistry and Biotechnology 77-79(9):689-699.