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Thomas C. Squier, Ph.D.


Tom Squier
Tom Squier

Dr. Squier's research centers on the development and application of molecular probes to visualize protein structrural dynamics in native biological environments using fluorescence spectroscopy. Newly developed probes permit the detection of structural heterogeneity and functional readouts of proteins tagged with small binding sequences in living cells. These measurements are important in understanding the basis for molecular recognition and catalysis, and offer a means to collect the necessary functional data in response to environmental change for the construction of predictive cellular models.Dr. Squier has held a variety of academic appointments and currently serves at Washington State University as an adjunct professor of biology.


  • Postdoc, Biophysics, University of Maryland, 1988-1990
  • Postdoc, Biochemistry, University of Maryland, 1985-1988
  • Ph.D., Biochemistry, University of Minnesota, 1985
  • A.B., Biology/Chemistry, Oberlin College, 1979

Awards and Honors

  • Ad-hoc Reviewer for National Institutes of Health (CCHF and NCI) 2004-present
  • Reviewer for American Heart Association (Cell Function and Metabolism) 2004-present
  • Reviewer for American Heart Association (Cellular Physiology) 2000-2004
  • Ad-hoc Reviewer for National Institutes of Health (BBCB) 2000
  • Editorial Board Member for Frontiers in Biosciences 1998-2003
  • Postdoctoral Fellowship from National Institutes of Health 1988-1990
  • Postdoctoral Fellowship from American Heart Association 1985-1988

Selected Publications

Yan P, T Wang, G Newton, TV Knyuschko, Y Xiong, DJ Bigelow, TC Squier, and MU Mayer. 2009. "A Targeted Releasable Affinity Probe (TRAP) for In Vivo Photocrosslinking." ChemBiochem 10, 1507-1518.

Chen TL, Q Yao, TA Soares, TC Squier, and DJ Bigelow. 2009. "Phospholamban modulates the conformational coupling between nucleotide domains in Ca-ATPase oligomeric complex in cardiac sarcoplasmic reticulum." Biochemistry, 48, 2411-2421.

Smallwood HS, D Lopez-Ferrer, PE Eberlein, DJ Watson, and TC Squier. 2009. "Calmodulin mediates DNA repair pathways involving H2AX in response to radiation exposure of RAW 264.7 macrophages." Chem. Res. Toxicol., 22, 460-470.

Chen B, JE Mahaney, MU Mayer, DJ Bigelow, and TC Squier. 2008. "Concerted but noncooperative activation of nucleotide and actuator domains of the Ca-ATPase upon calcium binding." Biochemisty 47, 12448-12456.

Chen B, DF Lowry, MU Mayer, and TC Squier. 2008. "Helix A stabilization preceeds amino-terminal lobe activation upon calcium binding to calmodulin." Biochemistry 46, 9220-9226.

Contact Information

Systems Biology at PNNL

Research & Capabilities