Jeffrey S. McLean

Expertise

Jeff McLean

Jeffrey S. McLean is currently a scientist at the Pacific Northwest National Laboratory in the Biological Sciences Division supporting Department of Energy programs such as the Genomes to Life and the laboratory-funded Biomolecular Systems Initiative. Jeff is currently leading projects focused on microbial biofilms which are attached communities of bacteria having environmental and medical relevance. These projects include the development of unique non-invasive, high resolution imaging and spectroscopy capabilities for biofilm applications. He is a co-principal investigator in projects involving the spatial and temporal metabolite profiling in live biofilms using a novel combined optical/magnetic resonance microscope, applying acoustic micro-ultrasound imaging to quantify biofilm architecture and material properties as well as the development and use of highly controlled cultivation systems for biofilm growth. He has also worked towards developing and operating the Microbial Cell Dynamics Laboratory (MCDL). This facility has 16 dedicated bioreactors and unique associated sensors and analytical equipment. In addition, his professional training has been in Confocal Laser Scanning Microscopy (CLSM), bioreactor operation and Fluorescence Activated Cell Sorting (FACS).

Education

M.S., Microbiology, University of Guelph, Canada, 2000
B.S., Environmental Geoscience, University of Toronto, Canada, 1997

Scholarships and Awards

PNNL Outstanding Performance Award, April 2002
On the Spot Award, June 2002
NSERC Industrial Postgraduate Scholarship, 1997, 1998, 1999
Latornell Travel Award, 1998
Guelph University Graduate Scholarship, 1997, 1998

Selected Publications

Majors PD, McLean JS, Pinchuk GE, Fredrickson JK, Gorby YA, Minard KR, and Wind RA. (2005). "NMR methods for in-situ biofilm metabolism studies." Journal of Microbiological Methods 62(3): 377-344.

Majors PD, McLean JS, Fredrickson JK, and Wind RA. (2005). “NMR methods for in-situ biofilm metabolism studies: spatial and temporal resolved measurements.” Water Science & Technology 52(7): 7–12.

Wunschel DS, Hill EA, McLean JS, Jarman K., Gorby YA, Valentine N. (2005) "Effects of varied pH, growth rate and temperature using controlled fermentation and batch culture on Matrix Assisted Laser Desorption/Ionization whole cell protein fingerprints." Journal of Microbiological Methods. Sep;62(3):259-71.

Hu JZ, Wind RA, McLean JS, Gorby YA, Resch CT, and Fredrickson JK. (2004). “High-Resolution 1H NMR Spectroscopy of Metabolically Active Microorganisms Using Non- Destructive Magic Angle Spinning,” Spectroscopy 19(12), 98-103.

McLean JS and Beveridge TJ. 2001. “Chromate reduction by a pseudomonad isolated from a site contaminated with chromated copper arsenate.” Appl Environ Microbiol. 67(3):1076-84.

Contact Information

Jeff.Mclean@pnl.gov