Chiann-Tso Lin, Ph.D.
Experience
Chiann-Tso Lin
- High through-put (HTP)- isolation (pull down) of intracellular protein complexes using automation procedures on the Biomek FX (Biorobot), HTP-purification of over-expressed proteins using the same automation platform.
- HTP-purification of proteins using Qiagen Biorobot.
- Handling with radioactive substances, isotope labeling of peptide.
- Chemical synthesis and modification of bio-organic compounds. Application of spectrometric methods (nmr, ir, uv, fluorescence).
- Separation methods for chemical compounds and biomolecules (HPLC, FPLC, chromato-focusing, capillary electrophoresis [CE]).
- Membrane biochemistry (Sugar Transport, Transport kinetics, Affinity labeling, Isolation and separation of membrane components, Purification of Membrane Proteins, Reconstitution of proteins into proteoliposomes).
- Cell culture, transfection and expression.
- Immunological studies (design polyclonal antipeptide antibodies), immuno-labeling, immuno-precipitation, immuno-affinity chromatography.
- Interactions of small molecules(ligand) and membrane proteins.
- Structural and functioanl relationship of a membrane protein (sodium/glucose cotransport protein).
Education
- Postdoc., Biochemistry, Max-Planck Institute of Biophysics, 1975-1977
- Ph.D., Orgnanic Chemistry, University Braunschweig, 1974
- B.S./M.S., Chemistry, University of Braunschweig, 1970
Honors and Awards
- The GAB Business Services Grant-in-Aid for Research awarded by the New York Heart Association, 1983-1986
- The Arthur Andersen & Company Investigatorship for Research, awarded by the New York Heart Association, 1982-1985
- Co-investigatorship in the Grant for Research, awarded to Dr. R. Kinne by the National Research Council, West Germany, 1978-1980
- Fellowship for Research by Max-Planck Institute, Frankfurt, West Germany, 1974-1978
- Scholarship for foreign graduate students from the Germany Academic Exchange Services for foreign scholars, 1970-1974
Selected Publications
Markillie LM, C-T Lin*, JN Adkins, DL Auberry, EA Hill, BS Hooker, PA Moore, RJ Moore, L Shi, SH Wiley, and V Kery. 2005. "A simple protein complex purification and identification method suitable for high throughput mapping of protein interaction networks." Journal of Proteome Research.
Shi L, C-T Lin*, LM Markillie, TC Squier, and BS Hooker. 2005. "Over-expression of Multi-heme C-type Cytochromes." Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington. Bio Techniques 297:(38)297-299.
Xia X, JT Lin*, and RKH Kinne. 2003. "Binding of Phlorizin to the Isolated C-terminal Extramembranous Loop of the Na+/Glucose Cotransporter Assessed by Intrinsic Trytophan Fluorescence." Biochemistry 42, 6115-6120.
Lin JT*, J Komanec, D Homerova, and RKH Kinne. 1999. "Probing Transmembrane Topology of the High-Affiniy sodium/Glucose cotranspoter (SGLT1) with Histidine-tagged Mutants." J. Memb. Biology 170, 243-252.
Lin JT*, J Kormanec, Wehner, S Wielert-Badt, and RKH Kinne. 1988. "High-level Expression of Na+/ D-glucose Cotransporter (SGLT1) in a Stably Transfected Chinese Hamster Ovary Cell Line." Biochim. Biophys. Acta 1373, 309-320.
*CT Lin and JT Lin are the same author.