LOCUS pPNL9_ 4884 bp DNA circular 18-JUL-2006 SOURCE ORGANISM COMMENT The location of all elements are approximate. ...but are fairly close....within 20 bases, The gal1 promoter is taken from invitrogen location of sequence witin YPD1. The UrA 3 gene from a blast of yeast sequence data base. http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|416315799| COMMENT VNTDBDATE|416315799| COMMENT LSOWNER| COMMENT VNTNAME|pPNL9_| COMMENT VNTAUTHORNAME|Cheryl Baird| COMMENT VNTAUTHORTEL|509 372 6760| COMMENT VNTAUTHOREML|Cheryl.baird@pnl.gov| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast FEATURES Location/Qualifiers misc_feature 512..805 /vntifkey="21" /label=alpha\prepro\leader misc_feature 806..850 /vntifkey="21" /label=linker misc_feature 869..910 /vntifkey="21" /label=V5\epitope\tag misc_feature 920..937 /vntifkey="21" /label=6\his primer_bind 419..446 /vntifkey="28" /label=Gal1B\for\seq\primer /note="nested primer to sequence PNL 9 inserts" misc_recomb 788..824 /vntifkey="86" /label=Gap\repair\site\PNL9For misc_recomb 851..888 /vntifkey="86" /label=PNL9\gap\repair\site misc_feature 3131..4034 /vntifkey="21" /label=URA3\gene rep_origin 1381..2200 /vntifkey="33" /label=ColE1\origin misc_marker 2228..3085 /vntifkey="22" /label=Amp\R rep_origin 4225..4740 /vntifkey="33" /label=cen/ars promoter 6..456 /vntifkey="29" /label=Gal\1\promoter misc_feature 779..805 /vntifkey="21" /label=HA\tag primer_bind 987..1005 /vntifkey="28" /label=CYC1\Rev\primer primer_bind 966..989 /vntifkey="28" /label=30seqrev\primer primer_bind 419..442 /vntifkey="28" /label=Gal1\for\PCR\primer primer_bind 266..289 /vntifkey="28" /label=pnl20\PCRfor primer_bind 850..868 /vntifkey="28" /label=pn9\rev primer_bind 804..824 /vntifkey="28" /label=pnl9\for BASE COUNT 1346 a 1136 c 1009 g 1393 t ORIGIN 1 ctagtacgga ttagaagccg ccgagcgggt gacagccctc cgaaggaaga ctctcctccg 61 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc 121 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca 181 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa 241 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga 301 tctattaaca gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca 361 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa 421 tatacctcta tactttaacg tcaaggagaa aaaaccccgg atcggactac tagcagctgt 481 aatacgactc actataggga atattaagct tatgagattt ccttcaattt ttactgctgt 541 tttattcgca gcatcctccg cattagctgc tccagtcaac actacaacag aagatgaaac 601 ggcacaaatt ccggctgaag ctgtcatcgg ttactcagat ttagaagggg atttcgatgt 661 tgctgttttg ccattttcca acagcacaaa taacgggtta ttgtttataa atactactat 721 tgccagcatt gctgctaaag aagaaggggt atctctcgag aaaagagagg ctgaagctta 781 cccatacgac gttccagact acgctggtgg tggtggttct gctagcgcgg ccgcggcctc 841 aggggcctcc ggaattctag aacaacaggg taagcctatc cctaaccctc tcctcggtct 901 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatccta 961 gagggccgca tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc 1021 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt 1081 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac 1141 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc 1201 tcgaaggctt taatttgcaa gctgcggccc tgcattaatg aatcggccaa cgcgcgggga 1261 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 1321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 1381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc 1441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 1501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 1561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 1621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 1681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 1741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 1801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 1861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 1921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 1981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 2041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 2101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 2161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 2221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 2281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagcgc ttaccatctg 2341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 2401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 2461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 2521 gcaacgttgt tggcattgct acaggcatcg tggtgtcact ctcgtcgttt ggtatggctt 2581 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 2641 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 2701 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 2761 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 2821 gttgctcttg cccggcgtca atacgggata atagtgtatc acatagcaga actttaaaag 2881 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 2941 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 3001 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 3061 cgacacggaa atgttgaata ctcatactct tcctttttca atgggtaata actgatataa 3121 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt 3181 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc 3241 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc 3301 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt 3361 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc 3421 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac 3481 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat 3541 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat 3601 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac 3661 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa 3721 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga 3781 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc 3841 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg 3901 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg 3961 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg 4021 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata 4081 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc 4141 aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa 4201 ttgaaaagct agcttatcga tgggtccttt tcatcacgtg ctataaaaat aattataatt 4261 taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa 4321 aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta 4381 ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt 4441 gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt 4501 atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt 4561 gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt 4621 tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc 4681 caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc 4741 cgccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggg gctagggcgg 4801 tgggaagtgt aggggtcacg ctgggcgtaa ccaccacacc cgccgcgctt aatggggcgc 4861 tacagggcgc gtggggatga tcca //