LOCUS pPNL-6 6468 bp DNA circular 19-AUG-2005 DEFINITION Complementary copy of pPNL-6. SOURCE ORGANISM COMMENT The Not1 site was put in to the Xho1 site of pCTCON from Dane wittrup using the following oligo: TCGATGCGGCCGCA which destroys the Xho1 site. The last revision of this plasmid was 030203. There should be two C's after the Ecor1 site just before the Aga2 protein that were missing and are now there. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|292416232| COMMENT VNTDBDATE|383223496| COMMENT LSOWNER| COMMENT VNTNAME|pPNL-6| COMMENT VNTAUTHORNAME|Michael Feldhaus| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast FEATURES Location/Qualifiers misc_structure complement(1112..1126) /vntifkey="88" /label=linker misc_difference complement(1127..1138) /vntifkey="85" /label=Xa misc_structure complement(1139..1165) /vntifkey="88" /label=HA\tag promoter 1..820 /vntifkey="29" /label=GAL1-10 misc_marker 4390..5247 /vntifkey="22" /label=AmpR CDS complement(2537..3238) /vntifkey="4" /label=TRP1 terminator complement(1475..1767) /vntifkey="43" /label=terminator misc_feature complement(5275..6094) /vntifkey="21" /label=ColE1\ori insertion_seq complement(851..1109) /vntifkey="14" /label=AGA2 insertion_seq complement(1166..1231) /vntifkey="14" /label=linker primer_bind complement(1138..1157) /vntifkey="28" /label=pPNL6\rev misc_feature complement(3741..4256) /vntifkey="21" /label=cen6/ars4 misc_feature complement(1415..1447) /vntifkey="21" /label=c-myc\tag misc_feature complement(851..904) /vntifkey="21" /label=Aga2p\signal\peptide primer 622..645 /vntifkey="27" /label=PNL20\PCR\for\primer primer complement(1514..1541) /vntifkey="27" /label=PNL20PCR\rev\primer primer complement(1484..1509) /vntifkey="27" /label=pnl20\seq\Rev\primer misc_feature 1232..1414 /vntifkey="21" /label=stuffer misc_feature 1209..1229 /vntifkey="21" /label=1209bp\pnl9\for primer_bind 1519..1539 /vntifkey="28" /label=pPNL6\for /note="used for PCR and mutagenic pcr of scFv" BASE COUNT 1807 a 1495 c 1309 g 1857 t ORIGIN 1 cgacaggtta tcagcaacaa cacagtcata tccattctca attagctcta ccacagtgtg 61 tgaaccaatg tatccagcac cacctgtaac caaaacaatt ttagaagtac tttcactttg 121 taactgagct gtcatttata ttgaattttc aaaaattctt actttttttt tggatggacg 181 caaagaagtt taataatcat attacatggc attaccacca tatacatatc catatacata 241 tccatatcta atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta 301 aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag 361 tacggattag aagccgccga gcgggtgaca gccctccgaa ggaagactct cctccgtgcg 421 tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg 481 aacaataaag attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa 541 cctggcccca caaaccttca aatgaacgaa tcaaattaac aaccatagga tgataatgcg 601 attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta 661 ttaacagata tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt 721 cggtttgtat tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata 781 cctctatact ttaacgtcaa ggagaaaaaa ccccggatcg aattccctac ttcatacatt 841 ttcaattaag atgcagttac ttcgctgttt ttcaatattt tctgttattg cttcagtttt 901 agcacaggaa ctgacaacta tatgcgagca aatcccctca ccaactttag aatcgacgcc 961 gtactctttg tcaacgacta ctattttggc caacgggaag gcaatgcaag gagtttttga 1021 atattacaaa tcagtaacgt ttgtcagtaa ttgcggttct cacccctcaa caactagcaa 1081 aggcagcccc ataaacacac agtatgtttt taaggacaat agctcgacga ttgaaggtag 1141 atacccatac gacgttccag actacgctct gcaggctagt ggtggtggtg gttctggtgg 1201 tggtggttct ggtggtggtg gttctgctag ctgcggtggc ggcggtacta gcaaaatttc 1261 tcattttttg aaaatggaat ctttgaattt tattagagct catactccat atattaatat 1321 ttacaattgt gaaccagcta atccatctga aaaaaattct ccatctactc aatattgtta 1381 ttctattcaa tcttcccagg tcgactgcgg gggcggatcc gaacaaaagc ttatttctga 1441 agaagacttg taatagctcg gcggccgcat cgagatctga taacaacagt gtagatgtaa 1501 caaaatcgac tttgttccca ctgtactttt agctcgtaca aaatacaata tacttttcat 1561 ttctccgtaa acaacatgtt ttcccatgta atatcctttt ctatttttcg ttccgttacc 1621 aactttacac atactttata tagctattca cttctataca ctaaaaaact aagacaattt 1681 taattttgct gcctgccata tttcaatttg ttataaattc ctataattta tcctattagt 1741 agctaaaaaa agatgaatgt gaatcgaatc ctaagagaat tgagctccaa ttcgccctat 1801 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 1861 ggcgttaccc aacttaatcg ccttgcagca catcccccct tcgccagctg gcgtaatagc 1921 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 1981 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 2101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 2161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 2221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 2341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 2401 aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta 2461 cgcatctgtg cggtatttca caccgcaggc aagtgcacaa acaatactta aataaatact 2521 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt 2581 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa 2641 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg 2701 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc 2761 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga 2821 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact 2881 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat 2941 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg 3001 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg 3061 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat 3121 ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag 3181 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt 3241 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt 3301 cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa 3361 ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact 3421 gcaaagtaca catatattac gatgctgtct attaaatgct tcctatatta tatatatagt 3481 aatgtcgttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 3541 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 3601 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 3661 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 3721 tgataataat ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct 3781 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt 3841 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa 3901 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga 3961 aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat 4021 tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc 4081 aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt 4141 cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata 4201 tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca 4261 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 4321 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 4381 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 4441 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 4501 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 4561 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 4621 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 4681 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 4741 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 4801 acaacgatcg gaggaccgaa ggagctaacc gctttttttc acaacatggg ggatcatgta 4861 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 4921 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 4981 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 5041 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 5101 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 5161 gttatctaca cgacgggcag tcaggcaact atggatgaac gaaatagaca gatcgctgag 5221 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 5281 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 5341 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5401 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5461 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5521 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 5581 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5641 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa 5821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5881 acaggagagc gcacgaggga gcttccaggg gggaacgcct ggtatcttta tagtcctgtc 5941 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggccgagc 6001 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 6061 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 6121 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 6181 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 6241 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 6301 gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg 6361 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 6421 gccaagctcg gaattaaccc tcactaaagg gaacaaaagc tgggtacc //