High-Throughput Proteomics for Trauma Research with the Inflammation and the Host Response to Injury Consortium
Dave Camp, PNNL Contact
NIH Lead, Massachusetts General Hospital
Funding Agency: National Institutes of Health, National Institute of General Medical Science
The host response to trauma and burns is a highly complex biological and pathological process that depends critically upon the regulation of the human immuno-inflammatory response. Scientists whom are part of the large-scale High-Throughput for Trauma Research with the Inflammation and the Host Response to Injury Consortium project team at Pacific Northwest National Laboratory (PNNL) and collaborating institutions are applying high-throughput biological discovery tools to identify important dynamic relationships that regulate this response, with the expectation that this knowledge will ultimately impact the treatment of trauma and burn patients. We are developing a high-throughput liquid chromatography-Fourier transform ion cyclotron resonance (LC-FTICR) approach that will facilitate >1600 LC-MS longitudinal proteomic analyses of enriched subpopulations of blood leukocytes and blood plasma from trauma and burn patients per year on a dedicated instrument. We also are investigating transient post-translational protein modifications in relevant proteins to provide additional information on critical participants in the signal transduction pathways and networks.
Related Publications
Qian WJ, JM Jacobs, T Liu, DG Camp II, RD Smith. 2006. “Advances and challenges in liquid chromatography-mass spectrometry based proteomic profiling for clinical applications.” Molecular and Cellular Proteomics Electronic Publication.
Liu T, WJ Qian, MA Gritsenko, W Xiao, LL Moldawer, A Kaushal, ME Monroe, SE Varnum, RJ Moore, SO Purvine, RV Maier, RW Davis, RG Tompkins, DG Camp II, RD Smith. 2006. “High dynamic range characterization of the trauma patient plasma proteome.” Molecular and Cellular Proteomics Electronic Publication.
Liu T, WJ Qian, HM Mottaz, MA Gritsenko, AD Norbeck, RJ Moore, SO Purvine, DG Camp II, RD Smith. 2006. “Evaluation of multi-protein immunoaffinity subtraction for plasma proteomics and candidate biomarker discovery using mass spectrometry.” Molecular and Cellular Proteomics Electronic Publication.
Liu T, WJ Qian, MA Gritsenko, DG Camp II, ME Monroe, RJ Moore, RD Smith. 2005. “The human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.” Journal of Proteome Research 4(6):2070-2080.
Jacobs JM, JN Adkins, WJ Qian, T Liu, Y Shen, DG Camp II, RD Smith. 2005. “Utilizing human blood plasma for proteomic biomarker discovery.” Journal of Proteome Research 4(4):1073-1085.
Qian WJ, ME Monroe, T Liu, JM Jacobs, GA Anderson, Y Shen, RJ Moore, DJ Anderson, R Zhang, SE Calvano, SF Lowry, W Xiao, LL Moldawer, RW Davis, RG Tompkins, DG Camp II, RD Smith, R.D. 2005. “Quantitative proteome analysis of human plasma following in vivo lipopolysaccharide administration using 16O/18O labeling and the accurate mass and time tag approach”. Molecular and Cellular Proteomics 4(5):700-709.
Qian, WJ, JM Jacobs, DG Camp II, ME Monroe, RJ Moore, MA Gritsenko, SE Calvano, SF Lowry, W Xiao, LL Moldawer, RW Davis, RG Tompkins, RD Smith. 2005. “Comparative proteome analyses of human plasma following in vivo lipopolysaccharide administration multidimensional separations coupled with tandem mass spectrometry.” Proteomics 5:572-584.
