Transcriptome and Proteome Mapping of the Mouse Brain
Dave Camp, PNNL Contact
Desmond Smith, Collaborator, University of California Los Angeles School of Medicine
Funding Agency: National Institutes of Health, National Institute of Neurological Disorders and Stroke
A major goal for post-genomic biology is to understand how the one-dimensional genome gives rise to the staggering three-dimensional (3-D) complexity of the mammalian brain. To address this area of research, it is necessary to spatially map transcripts, proteins, and the networks they form in the brain at a genome-wide level. The role of researchers at Pacific Northwest National Laboratory (PNNL) in the Transcriptome and Proteome Mapping of the Mouse Brain project is to employ liquid chromatography-Fourier transform ion cyclotron resonance (LC-FTICR) in combination with voxelation to map protein expression patterns in the mouse brain at volumetric resolutions of 11 µL and 1 µL. When combined with transcript expression patterns in the mouse brain at a genome-wide scale, this information will lead to insights into translational and post-translational control in the brain. Subsequent mining and display of the proteomic data as well as the application of statistical techniques to the combined data are expected to provide an intellectual infrastructure to help us understand how the digital information encoded by the genome is transformed into the 3-D structure of the brain.
Related Publications
Sacksteder CA, WJ Qian, TV Knyushko, H Wang, MH Chin, G Lacan, WP Melega, DG Camp II, RD Smith, DJ Smith, TC Squier, DJ Bigelow. 2006. “Endogenously nitrated proteins in mouse brain: Links to neurodegenerative disease.” Biochemistry 45(26):8009-8022.
Wang H, WJ Qian, MH Chin, VA Petyuk, RC Barry, T Liu, MA Gritsenko, HM Mottaz, RJ Moore, DG Camp II, AH Khan, DJ Smith, and RD Smith. 2006. “Characterization of the mouse brain proteome using global proteomic analysis complemented with cysteinyl-peptide enrichment.” Journal of Proteome Research 5(2):361-369.
Wang H, WJ Qian, HM Mottaz, TRW Clauss, DJ Anderson, RJ Moore, DG Camp II, AH Khan, DM Sforza, M Pallavicini, DJ Smith, RD Smith. 2005. “Development and evaluation of a micro- and nano-scale proteomic sample preparation method.” Journal of Proteome Research 4(6):2397-2403.
