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Mass Spectrometry

11.5 Tesla Fourier transform ion cyclotron resonance mass spectormeter
Pacific Northwest National Laboratory’s 11.5-Tesla Fourier transform ion cyclotron resonance mass spectrometer is used for biochemical applications, including protein characterization and proteomic research.

Mass spectrometry instrumentation at the Pacific Northwest National Laboratory (PNNL) is centrally located in the High Perfomance Mass Spectrometry (HPMS) Facility, which is part of the William R. Wiley Environmental Molecular Science Laboratory (EMSL). The HPMS facility provides state-of-the-art spectrometers coupled with electrospray ionization technology and fully integrated database capabilities.

Instrumentation in the facility has been refined for leading-edge analysis of biological problems and is suitable for biochemical and chemical analysis, separations research, mass spectrometry and molecular biology efforts.

Instrumentation and Applications

Fourier Transform Ion Cyclotron Resonance (FTICR) Spectrometers

  • 11.5-tesla, 205-mm bore spectrometer with Odyssey data station
  • 9.4-tesla, 160-mm bore APEX III spectrometer with high-throughput capability
  • 7-tesla, 150-mm bore spectrometer with Odyssey data station
  • 3.5-tesla, 330-mm bore spectrometer with Odyssey data station

Ion Trap and Quadrupole Spectrometers

  • Finnigan LCQ XPDECA Ion Trap Mass Spectrometer
  • Two Finnigan LCQ Classic Ion Trap Mass Spectrometers
  • Two Finnigan LCQ DUO Ion Trap Mass Spectrometer
  • Finnigan MAT TSQ 7000 Triple Quadrupole Mass Spectrometer
  • Sciex QSTARR Time-of-Flight Quadrupole Spectrometer, with a MicroIonSprayTM source

Additional Resources

  • Liquid Chromatography
  • Capillary Electrophoresis and Ionization Mass Spectrometry
  • Biochemical Sample Preparation and Support Capabilities
  • PAGE Electrophoresis

FTICR Software

PNNL-developed software ICR-2LS supports advanced experimental techniques and automated data analysis. Capabilities include:

  • Automated Mass Spectra Interpretation and Database Searching
  • Integration of FASTA or GenBank Files for Searching

Systems Biology at PNNL

Research & Capabilities

Resources

Proteomic tools at PNNL

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