Tangential Flow Filtration System
Maximate. Photo courtesy of the Pall Corporation.
Centramate. Photo courtesy of the Pall Corporation.
Cell Harvesting (0.2 µm, 300 kd molecular weight cut-off membrane filters are available at the MCDL)
Subcellular fraction and protein harvesting (300 kd, 100 kd molecular weight cut-off membrane filters are available at the MCDL)
Tangential flow filtration (TFF) is a rapid and efficient method for biomolecule separation. It can be applied to a wide range of biological fields such as immunology, protein chemistry, molecular biology, biochemistry, and microbiology. Pacific Northwest National Laboratory's Microbial Cell Dynamics Laboratory (MCDL) TFF system can fractionate and desalt solutions of samples ranging in size from one liter to hundreds of liters and concentrate them to as low as ten milliliters in a short period of time. Microfiltration membranes, with a pore size between 0.1 and 10 µm, are typically used for clarification, sterilization, removal of microparticulates, or for cell harvests. Currently the MCDL uses the Bioflow 5000 to cultivate cells up to 90 liters followed by TFF in tandem mode to concentrate 90 liters to 300 milliliters for harvesting outer membrane vesicles.
Visit the Manufacturer's Website for more detailed specifications.