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Microscopy

Spectral Confocal Microscope

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(MOV, 449KB) Three-dimensional reconstruction of Shewanella oneidensis MR-1 expressing green fluorescent protein attached to an iron oxide surface.
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(MOV, 607KB) Three-dimensional reconstruction of a suspended floc of Shewanella oneidensis MR-1 expressing green fluorescent protein stained with an exopolysaccharide stain (red).

With the unique capabilities of the spectral confocal microscope system at Pacific Northwest National Laboratory (PNNL), low-emission fluorescent compounds such as green fluorescent protein (gfp) are routinely used to probe biofilms and bacterial attachment processes in real-time. Three-dimensional (3-D) reconstruction of images is performed rapidly giving a complete view of the sample and the localization of the probes. This system has the best detection and resolution available with a patented prism spectrophotometer detector. Three laser lines are routinely used (488, 543, and 633) with a K-scanner at 2000 lines/second. The Leica TCS SP2 is the ideal confocal system because of the unique capabilities and the advanced integration between the microscope optics and powerful configurable software. The system is adaptable to a lot of expanding features such as Multiphoton, UV lasers, and simple inverted to upright microscope changeover.

Spectral Confocal Microscope - the Leica TCS SP2Spectral confocal microscope system. Photo courtesy of Leica Microsystems.

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