Cultivation and Processing Equipment
The creation of high-quality samples needed for a systems biology approach is achieved at Pacific Northwest National Laboratory (PNNL) using bioreactor systems under highly controlled continuous culture conditions. With precise control and monitoring of parameters such as dissolved oxygen, pH, and agitation, the growth rates of organisms can be set to produce homogeneous populations of cells for analyses of their proteome, transcriptome, metabalome, and physiology. The highly adaptable BioFlo 3000® system allows Microbial Cell Dynamics Laboratory (MCDL) users to run chemostat or batch cultures in 1.5, 2.5, 5, and 10L vessels. With remote control capability and many controlled parameters available, these reactors are fundamental to the creation of controlled and biologically relevant samples.
The MCDL currently has two of these bioreactors.
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